Coding

Part:BBa_K4810004:Design

Designed by: Theodoros Desiniotis, Ioannis Stamelos, Aikaterini Spanou   Group: iGEM23_Athens   (2023-10-10)


Modified M13 pVIII protein for Eicosane binding


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original sequence consists of 222bp coding for 73 amino acids. First 23 amino acids are cleaved by a signal-peptidase inside E.coli cells. Thus in order to introduce the modified hexapeptide (or any peptide) in the N-terminal of the functional protein, the appropriate codons should be introduced from the codon #24 and onwards in the sequence of the pVIII protein.



Source

unmodified sequence from M13KE genome

References